Nanopore Sequencing
Nanopore Sequencing Services
SeqCenter is a Certified Provider of Oxford Nanopore Sequencing.
We use the latest PCR-free ligation library preparation (V14 chemistry) that provides reads equal to the length of the input DNA, which have been shown to assemble into complete genomes with as little as 12x coverage. All sequencing is performed on R10.4.1 flowcells run on a GridION platform and basecalling is included using the most up to date version of Guppy run is Super High Accuracy mode (with methylation pattern recognition) to achieve near Q20 performance.
For each submitted sample, you will receive the raw data in both fast5 and fastq format, as well as BAM files with 5mC calls and assembly in fasta format.
Nanopore Sample Requirements:
- Total volume of 60µL or more
- Eluted into water or dilute Tris/TE buffer
- Sample concentration of greater than:
- 40ng/µL measured by Qubit
- 100ng/µL measured by Nanodrop
What You Will Receive:
- Fastq file for each sample
- BAM file with 5mC Calls
- Fasta assembly file for each sample
- Materials and Methods Summary
- Data shared via Box folder within 2 weeks
Offering | Description | Price* |
|---|---|---|
300Mbp | Minimum of 300Mbp Nanopore Reads Only | $280.00 |
600Mbp | Minimum of 600Mbp Nanopore Reads Only | $335.00 |
1.2Gbp | Minimum of 1.2Gbp Nanopore Reads Only | $450.00 |
Dedicated NP Flowcell | Full GridION Flowcell, Total Output 10-20Gbp | $1,200.00 |
*Orders >48 Samples receive 5% discount
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Additional Options for Nanopore: HMW DNA Extractions
HMW Genomic DNA isolation is available at a rate of $20 per sample
- Extractions will be performed using Zymo Quick-DNA HMW MagBead Kits
- We do not accept live BSL3 or above samples for extractions.
- Samples should be sent as sealed pre-grown agar plates or aspirated frozen cell pellets (10^9 cells recommended).
Hybrid Assembly Sequencing
When used in combination with Illumina reads (to improve accuracy at the nucleotide level), long read sequencing is a perfect choice for closing genomes and assembling communities. Our facility uses the latest, proven software to combine the two data types into a single, annotated genome file. Samples sequenced under these packages will receive both the Nanopore and Illumina raw reads, as well as the assembled and annotated genome in .fasta, .gbk and .gff format.
Hybrid Assembly Sample Requirements:
- Samples must be submitted in 2 tubes for Illumina and Nanopore sequencing
- Eluted into water or dilute Tris/TE buffer
- Sample concentration matching requirements for Illumina and Nanopore sequencing
What You Will Receive:
- 3 Fastq files for each sample, corresponding to Illumina and Nanopore raw data
- BAM file with 5mC Calls
- Assembled genome in .fasta format and annotated in .gff and .gbk formats
- Assembly Metrics and Summary
- Materials and Methods Summary
- Data shared via Box folder within 2 weeks
Sequencing Package | Description | Price* |
|---|---|---|
Small Nanopore Combo | Minimum 300Mbp Long Reads, 400Mbp Illumina Reads, Genome Assembly and Annotation | $400.00 |
Medium Nanopore Combo | Minimum 600Mbp Long Reads, 1Gbp Illumina Reads, Genome Assembly and Annotation | $500.00 |
Large Nanopore Combo | Minimum 1.2Gbp Long Reads, 2Gbp Illumina Reads, Genome Assembly and Annotation | $650.00 |
*Orders >48 Samples receive 5% discount
PLEASE SEE OUR FAQ FOR MORE INFORMATION ABOUT MEASUREMENT METHODS