PacBio Sequencing
PacBio Whole Genome Sequencing
SeqCenter PacBio HiFi services can accommodate organisms of any size on the Sequel IIe platform. We recommend PacBio services for large-scale, eukaryotic genomes (amphibians, birds, reptiles, mammals, etc.) at a target depth of coverage between 15-20x.
Using Megaruptor 3 technology and PacBio’s SMRTbell v3.0 library preparation kit, sample gDNA is fragmented to the 15-18kb range (or longer by request) and sequenced using the latest PacBio SMRTbell chemistry.
Each flowcell includes 1 library preparation. If you are interested in multiplexing additional samples per flowcell, please contact us. Additional library preparations cost $250.00 per sample.
All HiFi PacBio services come with a complimentary collapsed-genome assembly (via Flye) and annotation*. Partially or fully-phased** assembly services (via Hifiasm) can replace the complimentary collapsed-genome assembly upon request.
Our HMW DNA Extraction add-on service is highly recommended as PacBio sequencing is highly sensitive to sample degradation and freshly extracted DNA produces the best results. If this option is not selected, please ship frozen samples on dry ice.
*Annotation is conditional to database availability and requires a genus/species NCBI taxonomic ID.
**Fully-phased assemblies via Hifiasm (trio-binning) require parental Illumina short reads. Externally generated short read data may be subject to fees if reformatting is required for analysis.
PacBio Sample Requirements:
- Total volume of 60µL or more
- Concentration greater than 35ng/uL as measured by Qubit or other fluorimetric method (not Nanodrop)
- Average fragment size greater than 15kb
- Eluted into water or dilute Tris/TE buffer
What You Will Receive:
- Raw Fastq file for each sample
- Assembly for each sample
- Materials and Methods Summary
- Data shared via Box folder within 2 weeks.
Offering | Description | Price |
|---|---|---|
Full 8M Flowcell x 1 | Includes 1 Library Prep Run Across a Full Flowcell | $2,900.00 |
Full 8M Flowcell x 2 | Includes 1 Library Prep Run Across 2 Full Flowcells | $4,900.00 |
Full 8M Flowcell x 3 | Includes 1 Library Prep Run Across 3 Full Flowcells | $6,900.00 |
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Additional Options for PacBio: HMW DNA Extractions
HMW Genomic DNA isolation is available at a rate of $20 per sample
- Extractions will be performed using Zymo Quick-DNA HMW MagBead Kits
- We do not accept live BSL3 or above samples for extractions.
- Samples should be sent as sealed pre-grown agar plates or aspirated frozen cell pellets (10^9 cells recommended).
Custom PacBio Sequencing Projects
PacBio sequencing technology unlocks a large number of applications aside from whole genome assemblies and we are able to take on projects that are tailored to your needs. Our most popular projects include full length AAV Sequencing, MAS-Seq of 10x Chromium libraries, and CCS services.
Please reach out to us with additional project details for pricing.
PLEASE SEE OUR FAQ FOR MORE INFORMATION ABOUT MEASUREMENT METHODS