Quote #:
Order #:
Purchase Date: 01/01/70
Payment Method:
Customer Name:
Institution:
Email:
Phone:
Address:
Bill to:
Sample Details
# | Sequence Offering | Sample Name | Sample Concentration ng/uL |
---|
Overall Notes
When submitting your samples please ensure they meet the following requirements:
- All purified nucleic acid sample should be resuspended in nuclease-free water or dilute Tris or TE buffer. (Most extraction kit buffers are OK.)
- Samples should be sent in microcentrifuge tubes labeled with permanent marker or sealed 96-well PCR plates for larger submissions. (Please send PCR plates on dry ice to reduce the possibility of leaking or cross contamination.)
- We ask customers to not use sticker labels, as they are prone to fall off.
- When shipping with dry ice, include at least 5-10lbs of dry ice per day of expected transit. Do NOT ship Thursday or Friday.
- Concentration values below correspond to Qubit or other dye-based methods. If using a Nanodrop, at least double is required.
Extractions
Generally, 109 cells are required either as aspirated cell pellets, well-grown lawns on agar, or homogenized tissue. For exact requirements, see the extraction section of the specific service page.
DNA, Illumina Material Requirements
WGS, WES, and Shotgun Metagenome | 16S(V3-V4)/ITS2 | Full Flowcells | Hybrid Sequencing | |
---|---|---|---|---|
Volume | ≥ 30 µL | ≥ 20 µL | ≥ 30 µL, single pool | Two aliquots are required: 1 meeting Illumina WGS reqs. 1 meeting ONT Ligation reqs. |
Qubit quantification | ≥ 10 ng/µL | No minimum | ≥ 1 ng/µL, final pool | |
Shipping conditions | Ambient | Ambient | Dry ice | |
Other | Fragment length ≥ 400 bp | If using custom primers, please send 10-20 µL at 100 µM of each. |
DNA, Long Read Material Requirements
ONT Ligation | Nanopore Ultra-Long | PacBio WGS | Full-Length 16S | |
---|---|---|---|---|
Volume | ≥ 60 µL | 750 µL | ≤ 100 µL | ≥ 20 µL |
Qubit quantification | ≥ 40 ng/µL | ≥ 40 ng/µL | ≥ 2 µg total | ≥1 ng/µL |
Shipping conditions | Cool packs or dry ice, overnight recommended | |||
Other | Must have no contact with Axygen consumables |
RNA Material Requirements
Illumina RNA and Metatranscriptomics | Nanopore Direct RNA (poly-A only) | PacBio Iso-Seq (poly-A only) | |
---|---|---|---|
Volume | ≥ 20 µL | ≥ 20 µL | ≤ 7 µL |
Qubit quantification | ≥ 50 ng/µL | ≥ 15 ng/µL - pre-depleted ≥ 50 ng/µL - undepleted |
≥ 500 ng mRNA total |
Shipping conditions | Dry ice, overnight required | ||
RIN Recommendation | > 6 | > 8 | > 7 |
Other | Must have no contact with Axygen consumables. |
Specialized Service Material Requirements
PacBio AAV | Illumina SARS-CoV-2 | |
---|---|---|
Volume | ≤ 50 µL | ≥ 15 µL, RNA or ≥ 250 µL whole sample in VTM |
Qubit quantification | ≥ 500 ng total | None, but CT value < 35 |
Shipping conditions | Dry ice, overnight required | |
Other | Must have no contact with Axygen consumables. |
If your samples are unable to meet these requirements due to the nature of your organism, please contact us to see if custom library preparation is available.
*Please our FAQ for more information about the differences in Qubit and Nanodrop measurements.
Shipping Information:
Please send samples to:
SeqCenter
91 43rd Street, Suite 250
Pittsburgh, PA 15201, USA
For additional shipping questions, please contact us at info@seqcenter.com