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Purchase Date: 01/01/70

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All Results in 2 Weeks or Less


Measurement Method:

Sample Details

# Sequence Offering Sample Name Sample Concentration ng/uL

Overall Notes

When submitting your samples please ensure they meet the following requirements:

  • All purified nucleic acid sample should be resuspended in nuclease-free water or dilute Tris or TE buffer. (Most extraction kit buffers are OK.)
  • Samples should be sent in microcentrifuge tubes labeled with permanent marker or sealed 96-well PCR plates for larger submissions. (Please send PCR plates on dry ice to reduce the possibility of leaking or cross contamination.)
  • We ask customers to not use sticker labels, as they are prone to fall off.
  • When shipping with dry ice, include at least 5-10lbs of dry ice per day of expected transit. Do NOT ship Thursday or Friday.
  • Concentration values below correspond to Qubit or other dye-based methods. If using a Nanodrop, at least double is required.

Extractions

Generally, 109 cells are required either as aspirated cell pellets, well-grown lawns on agar, or homogenized tissue. For exact requirements, see the extraction section of the specific service page.

DNA, Illumina Material Requirements

WGS, WES, and Shotgun Metagenome 16S(V3-V4)/ITS2 Full Flowcells Hybrid Sequencing
Volume ≥ 30 µL ≥ 20 µL ≥ 30 µL, single pool Two aliquots are required: 1 meeting Illumina WGS reqs. 1 meeting ONT Ligation reqs.
Qubit quantification ≥ 10 ng/µL No minimum ≥ 1 ng/µL, final pool
Shipping conditions Ambient Ambient Dry ice
Other Fragment length ≥ 400 bp If using custom primers, please send 10-20 µL at 100 µM of each.

DNA, Long Read Material Requirements

ONT Ligation Nanopore Ultra-Long PacBio WGS Full-Length 16S
Volume ≥ 60 µL 750 µL ≤ 100 µL ≥ 20 µL
Qubit quantification ≥ 40 ng/µL ≥ 40 ng/µL ≥ 2 µg total ≥1 ng/µL
Shipping conditions Cool packs or dry ice, overnight recommended
Other Must have no contact with Axygen consumables

RNA Material Requirements

Illumina RNA and Metatranscriptomics Nanopore Direct RNA (poly-A only) PacBio Iso-Seq (poly-A only)
Volume ≥ 20 µL ≥ 20 µL ≤ 7 µL
Qubit quantification ≥ 50 ng/µL ≥ 15 ng/µL - pre-depleted
≥ 50 ng/µL - undepleted
≥ 500 ng mRNA total
Shipping conditions Dry ice, overnight required
RIN Recommendation > 6 > 8 > 7
Other Must have no contact with Axygen consumables.

Specialized Service Material Requirements

PacBio AAV Illumina SARS-CoV-2
Volume ≤ 50 µL ≥ 15 µL, RNA or ≥ 250 µL whole sample in VTM
Qubit quantification ≥ 500 ng total None, but CT value < 35
Shipping conditions Dry ice, overnight required
Other Must have no contact with Axygen consumables.

If your samples are unable to meet these requirements due to the nature of your organism, please contact us to see if custom library preparation is available.

*Please our FAQ for more information about the differences in Qubit and Nanodrop measurements.

Shipping Information:

Please send samples to:
SeqCenter
91 43rd Street, Suite 250
Pittsburgh, PA 15201, USA

For additional shipping questions, please contact us at info@seqcenter.com

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