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Purchase Date: 01/01/70

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All Results in 2 Weeks or Less

Measurement Method:

Sample Details

# Sequence Offering Sample Name Sample Concentration ng/uL

Overall Notes

When submitting your samples please ensure they meet the following requirements:

  • All purified nucleic acid sample should be resuspended in nuclease-free water or dilute Tris or TE buffer. (Most extraction kit buffers are OK.)
  • Samples should be sent in microcentrifuge tubes labeled with permanent marker or sealed 96-well PCR plates for larger submissions. (Please send PCR plates on dry ice to reduce the possibility of leaking or cross contamination.)
  • We ask customers to not use sticker labels, as they are prone to fall off.
  • When shipping with dry ice, include at least 5-10lbs of dry ice per day of expected transit. Do NOT ship Thursday or Friday.
  • Concentration values below correspond to Qubit or other dye-based methods. If using a Nanodrop, at least double is required.

Extractions

Generally, 109 cells are required either as aspirated cell pellets, well-grown lawns on agar, or homogenized tissue. For exact requirements, see the extraction section of the specific service page.

DNA, Illumina Material Requirements

WGS, WES, and Shotgun Metagenome 16S(V3-V4)/ITS2 Full Flowcells Hybrid Sequencing
Volume ≥ 30 µL ≥ 20 µL ≥ 30 µL, single pool Two aliquots are required: 1 meeting Illumina WGS reqs. 1 meeting ONT Ligation reqs.
Qubit quantification ≥ 10 ng/µL No minimum ≥ 1 ng/µL, final pool
Shipping conditions Ambient Ambient Dry ice
Other Fragment length ≥ 400 bp If using custom primers, please send 10-20 µL at 100 µM of each.

DNA, Long Read Material Requirements

ONT Ligation Nanopore Ultra-Long PacBio WGS Full-Length 16S
Volume ≥ 60 µL 750 µL ≤ 100 µL ≥ 20 µL
Qubit quantification ≥ 40 ng/µL ≥ 40 ng/µL ≥ 2 µg total ≥1 ng/µL
Shipping conditions Cool packs or dry ice, overnight recommended
Other Must have no contact with Axygen consumables

RNA Material Requirements

Illumina RNA and Metatranscriptomics Nanopore Direct RNA (poly-A only) PacBio Iso-Seq (poly-A only)
Volume ≥ 20 µL ≥ 20 µL ≤ 7 µL
Qubit quantification ≥ 50 ng/µL ≥ 15 ng/µL - pre-depleted
≥ 50 ng/µL - undepleted		 ≥ 500 ng mRNA total
Shipping conditions Dry ice, overnight required
RIN Recommendation > 6 > 8 > 7
Other Must have no contact with Axygen consumables.

Specialized Service Material Requirements

PacBio AAV Illumina SARS-CoV-2
Volume ≤ 50 µL ≥ 15 µL, RNA or ≥ 250 µL whole sample in VTM
Qubit quantification ≥ 500 ng total None, but CT value < 35
Shipping conditions Dry ice, overnight required
Other Must have no contact with Axygen consumables.

If your samples are unable to meet these requirements due to the nature of your organism, please contact us to see if custom library preparation is available.

*Please our FAQ for more information about the differences in Qubit and Nanodrop measurements.

Shipping Information:

Please send samples to:
SeqCenter
91 43rd Street, Suite 250
Pittsburgh, PA 15201, USA

For additional shipping questions, please contact us at info@seqcenter.com

Contact:
91 43rd Street, Ste. 250
Pittsburgh, PA 15201
(878) 227-4915

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Full Flowcell:

We offer full flowcell sequencing services for libraries that have been prepared outside of our lab. For each library, we will need to know the loading concentration, phiX spike-in, and run structure. Data is delivered as a complete run folder and can be demultiplexed upon request if a sample sheet is provided.

SARS-CoV-2 Sequencing:

Our SARS-CoV-2 Sequencing services include an assembly on the SARS-CoV-2 reference genome as a scaffold. These packages provide roughly 200Mbp of paired end reads (2×151 bp) as fastq files and the assembly in fasta format.

We require a minimum sample volume of 15 µL of extracted material (or 250µL for VTM samples) and recommend that only samples with Ct values below 35 are sent (because submissions are not measured for viral load before processing). We offer services for both purified RNA and samples in VTM media that require viral RNA extraction. Please see our SARS-CoV-2 Sequencing page for more detailed information.

Microbiome Sequencing:

Please see our Microbiome Sequencing page for package details, including distributables and sample requirements.

Nanopore Sequencing:

Our Oxford Nanopore sequencing packages provide reads equal to the length of input DNA, delivered as a fastq file.

For each sample, we require a minimum volume of 60 µL, and at least 40 ng/µL dsDNA quantified by Qubit or other dye-based method (100 ng/µL by Nanodrop). Variant calling add-on analysis requires an annotated reference file. DNA extraction services are available for only specific categories of microbes.

Please see our DNA Sequencing: Long Read Sequencing page for more detailed information about the package, variant calling analysis, and extraction requirements.

PacBio:

Our PacBio services deliver HiFi reads with an average read length of 8-12kb for standard offerings and up to 25kb for full flowcell runs. All package names are listed by the minimum number of >Q20 reads that will be delivered. Both assembly and annotation are included with all packages.

For each sample, a minimum volume of 60 µL and at least 35 ng/µL dsDNA (quantified by Qubit or other dye-based methods) are required. SeqCenter strongly recommends utilizing our HMW DNA Extraction service for best results; however, we will also accept samples that are shipped on dry ice to avoid DNA integrity issues caused by the shipping process.

Please see our PacBio Sequencing page for more detailed information about our services.

RNA Sequencing:

We offer both rRNA depletion RNA squencing and polyA-tail enrichment mRNA sequencing. Both provide stranded, paired end reads (2x 151bp) as fastq files.

For each sample, we require a minimum volume of 20µL and at least 50ng/µL RNA. We also recommend, but do not require, that samples be DNAse treated and have a RIN score greater than 6. We also offer Basic and Intermediate RNAseq analyses, which require an annotated reference sequence.

Please see our RNA Sequencing page for more detailed information about both our RNA sequencing and analysis packages.

Illumina Sequencing:

The Illumina sequencing packages produce paired end reads (2×151 bp) delivered as fastq files.

For each sample, we require a minimum volume of 30 µL and at least 10 ng/µL dsDNA quantified by Qubit or other dye-based method (20 ng/µL by Nanodrop). Variant calling add-on analysis requires an annotated reference file. DNA extraction services are available for only specific categories of microbes.

Please see our DNA Sequencing: Whole Genome Sequencing page for more detailed information about the package, variant calling analysis, and extraction requirements.

For additional information about variant calling and short read assemblies, please see our Bioinformatics page.

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