Reference Based Variant Calling
For only $10 per sample, we will take your SeqCenter generated Illumina data and compare it to an already assembled reference file (often retrieved from depositories such as NCBI) using a program called Breseq (for haploid organsims). The output of this comparison is a very user-friendly .html file that is navigable in any web browser even by novice users. To order this add-on, simply request it on your order form and provide the desired reference file (.dna and .snap files are not accepted).
Short Read Assembly
There is not always a good reference available for the organism that you are working with, but that is no longer a barrier to analysis. For $20 per sample, we will de novo assemble your haploid genome using the latest, proven assembly software. It is important to note that the short reads of Illumina sequencing are not ideal for assembly and will generally result in >100 contigs. For a better assembly, we recommend using Nanopore Combo sequencing services, which can be found here. To order this service, please contact us by email at firstname.lastname@example.org to discuss your samples and assembly needs.
We understand that there are countless tools available to analyze your sequencing data. If you have a project in mind that is more involved than our standard offerings, please reach out to us with a description of the work you would like performed and we will gladly provide you with a custom quote on a plan that will meet your needs.