You will receive your invoice within 2 business days of completion of your order and delivery of your data. If you require an invoice in a specific timeframe, please send a request to email@example.com. We are happy to discuss your funding deadline and accommodations we may be able to make.
When you entered your credit card information through our online ordering system, an authorization was run, and a temporary hold was placed on your card in the amount that was quoted, plus a 3% processing fee.
This hold expires after 27 days or until we capture the funds. We do not capture funds until we invoice, which is done after we complete your order and deliver your data. At this time, an invoice/receipt is emailed to the billing contact as well as to the person who placed the order.
Unfortunately, we do not offer academic or new customer discounts. We implement methods that allow us to offer some of the lowest prices in the industry. Instead of offering occasional discounts, we take pride in keeping our prices as low as possible throughout the year to make sequencing consistently accessible to all researchers and budgets.
We accept valid Purchase Orders (PO) issued by your institution or credit card payments. There is a 3% processing fee for credit card transactions and a $20 wire transfer fee for international wire transfer payments.
POs are issued by a customer’s institution as a formal agreement to request specific quantities and types of services from SeqCenter and are a promise to pay for the services at the prices listed on the quote. Funds are only transferred when a formal invoice is submitted. For a customer’s institution, these also specify which funds or account are being used to pay. For SeqCenter, these are required for us to request payment when submitting an invoice after work is done. (Without a PO, we will not be paid.)
If SeqCenter has not done business with your institution before, we may be required to register as a vendor before a PO can be issued.
The bulk discount is meant to incentivize large shipments, which allow us to fill out sequencing runs more quickly. This allows us to deliver data to all customers even faster, and as such, we like to pass on the small discount to large volume shipments as a “thank you.”
We offer full flowcell sequencing services for libraries that have been prepared outside of our lab. For each library, we will need to know the loading concentration, phiX spike-in, and run structure. Data is delivered as a complete run folder and can be demultiplexed upon request if a sample sheet is provided.
Our SARS-CoV-2 Sequencing services include an assembly on the SARS-CoV-2 reference genome as a scaffold. These packages provide roughly 200Mbp of paired end reads (2×151 bp) as fastq files and the assembly in fasta format.
We require a minimum sample volume of 15 µL of extracted material (or 250µL for VTM samples) and recommend that only samples with Ct values below 35 are sent (because submissions are not measured for viral load before processing). We offer services for both purified RNA and samples in VTM media that require viral RNA extraction. Please see our SARS-CoV-2 Sequencing page for more detailed information.
Our Oxford Nanopore sequencing packages provide reads equal to the length of input DNA, delivered as a fastq file.
For each sample, we require a minimum volume of 60 µL, and at least 40 ng/µL dsDNA quantified by Qubit or other dye-based method (100 ng/µL by Nanodrop). Variant calling add-on analysis requires an annotated reference file. DNA extraction services are available for only specific categories of microbes.
Our PacBio services deliver HiFi reads with an average read length of 8-12kb for standard offerings and up to 25kb for full flowcell runs. All package names are listed by the minimum number of >Q20 reads that will be delivered. Both assembly and annotation are included with all packages.
For each sample, a minimum volume of 60 µL and at least 35 ng/µL dsDNA (quantified by Qubit or other dye-based methods) are required. SeqCenter strongly recommends utilizing our HMW DNA Extraction service for best results; however, we will also accept samples that are shipped on dry ice to avoid DNA integrity issues caused by the shipping process.
We offer both rRNA depletion RNA squencing and polyA-tail enrichment mRNA sequencing. Both provide stranded, paired end reads (2x 151bp) as fastq files.
For each sample, we require a minimum volume of 20µL and at least 50ng/µL RNA. We also recommend, but do not require, that samples be DNAse treated and have a RIN score greater than 6. We also offer Basic and Intermediate RNAseq analyses, which require an annotated reference sequence.
Please see our RNA Sequencing page for more detailed information about both our RNA sequencing and analysis packages.
The Illumina sequencing packages produce paired end reads (2×151 bp) delivered as fastq files.
For each sample, we require a minimum volume of 30 µL and at least 10 ng/µL dsDNA quantified by Qubit or other dye-based method (20 ng/µL by Nanodrop). Variant calling add-on analysis requires an annotated reference file. DNA extraction services are available for only specific categories of microbes.