RNA Sequencing
rRNA Depletion RNA Sequencing
Our lab utilizes Illumina Stranded RNA library preparation with RiboZero Plus rRNA depletion to offer RNA Sequencing at a very economical price point for a wide range of samples from bacteria to human to metatranscriptomes. This method captures both coding and non-coding RNA. Our dedicated RNA sequencing pipeline that provides a wide range of paired end reads (2x150bp) to suite your project needs.
We are unable to guarantee the compatibility of Illumina’s RiboZero Plus with Microbiome module with all microbial organisms, however, a list of tested fungal and bacterial organisms can be found here. If you are interested in supplementing the modules with probes designed specifically for your organism, please contact us at info@seqcenter.com for additional information.
All of our offerings include DNAse Treatment, rRNA Depletion, Stranded Library Prep and sequencing to requested amount.
RNA Sample Requirements:
- Samples shipped frozen on dry ice
- Total volume of 20µL or more
- Sample concentration greater than 50ng/uL
- DNAse Treated (Recommended, not required)
- RIN > 6 (Recommended measurement, not required)
What You Will Receive:
- Fastq files (2 x 150bp) for each sample
- Data QC Summary
- Materials and Methods Summary
- Data shared via Box folder within 2 weeks.
Sequencing Package (Minimum Read Count per Sample)* | Example Uses | Price* |
|---|---|---|
12M Paired End Reads (1.8Gbp) | Bacteria | $250.00 |
25M Paired End Reads (3.75Gbp) | Yeast | $275.00 |
50M Paired End Reads (7.5Gbp) | Larger Fungi, Fruit Flies, Algae | $350.00 |
100M Paired End Reads (15Gbp) | Mouse, Human, Simple Metatranscriptomes | $500.00 |
200M Paired End Reads (30Gbp) | Transcriptome Assembly, Diverse Metatranscriptomes | $700.00 |
mRNA Sequencing
SeqCenter utilizes Illumina Stranded mRNA library preparation with to provide an economical RNA sequencing option for organisms with polyA-tailed RNA transcripts. This service is appropriate for most eukaryotic studies and provides a minimum of the number of PE reads listed for each package (2x150bp).
All of our offerings include DNAse Treatment, mRNA (PolyA) Enrichment, Stranded Library Prep and sequencing to requested amount.
RNA Sample Requirements:
- Samples shipped frozen on dry ice
- Total volume of 20µL or more
- Sample concentration greater than 50ng/uL
- DNAse Treated (Recommended, not required)
- RIN > 6 (Recommended measurement, not required)
What You Will Receive:
- Fastq files (2 x 150bp) for each sample
- Data QC Summary
- Materials and Methods Summary
- Data shared via Box folder within 2 weeks.
Sequencing Package (Minimum Read Count per Sample)* | Example Uses | Price* |
|---|---|---|
25M Paired End Reads (3.75Gbp) | Yeast | $225.00 |
50M Paired End Reads (7.5Gbp) | Large Fungi, Fruit Flies, Algae | $300.00 |
100M Paired End Reads (15Gbp) | Mouse and Human | $450.00 |
200M Paired End Reads (30Gbp) | Transcriptome Assembly | $650.00 |
RNA Sequencing Analysis
The following are available as standard add-on analysis packages to any RNA Seq data that is generated by SeqCenter. An annotated reference genome (not .fasta) must be provided for analysis (.dna and .snap formats are not accepted). For ‘Intermediate RNA Analysis’ packages, SeqCenter can perform pathway analysis only with references that are included in the KEGG Pathway Catalog. Please verify in the order notes that the correct source is specified (GenBank/RefSeq) and the corresponding NCBI accession number that correlates to that specific version (GCA vs GCF.)
All prices are per sample, per reference (i.e. a dual-seq experiment will require two analyses per sample).
Analysis Package | Description of Deliverables | Price |
|---|---|---|
Basic | Provides raw transcript level quantification using user provided reference. Recommended for those experienced with RNA Seq data. | $20.00 |
Intermediate | Provides transcript level quantification, count normalization, differential expression analysis between treatment groups, global principal component analysis (PCA), and pathway analysis (when applicable). | $40.00 |
PLEASE SEE OUR FAQ FOR MORE INFORMATION ABOUT MEASUREMENT METHODS