Microbiome Sequencing
16S/ITS Sequencing
Our center utilizes Zymo Quick-16S Plus Library Prep methods to selectively amplify either the V3/V4 region (bacteria/archaea) or ITS2 region of your metagenomic sample. Using the NextSeq 2000 platform, this service delivers raw paired-end (2x301bp) as fastq files and preliminary analysis reports (no additional charge) to include Species Abundance Tables, Alpha and Beta Diversity, Taxonomic Assignment, and Diversity Barplots by Taxonomic Rank.
16S/ITS Sample Requirements:
- Total volume of 20µL or more
- Sample eluted into water or dilute Tris/TE buffer
- Sample concentration of greater than 10ng/uL
What You Will Receive:
- Fastq files (2 x 301bp) for each sample
- Data Analysis and QC Summary
- Materials and Methods Summary
- Data shared via Box folder within 2 weeks.
Sequencing Package | Price* |
|---|---|
20k Reads (10k Read Pairs) - Either V3/V4 or ITS2 Region | $45.00 |
50k Reads (25k Read Pairs) - Either V3/V4 or ITS2 Region | $55.00 |
100k Reads (50k Read Pairs) - Either V3/V4 or ITS2 Region | $75.00 |
*Orders >48 Samples receive 5% discount
Shotgun Metagenomics
Rather than target the the 16S gene region alone, our shotgun metagenomics approach sequences your sample’s entire community using Illumina DNA Library Prep methods. Community profiling is beneficial for users that are more interested in the functional gene content of their sample as opposed to the identification of the microbial genus and species that make it up. All samples include sequencing data as well as Metagenomic Assembly, Assembly Annotation, and Taxonomic Assignment. If analysis is not needed, please see our DNA sequencing options for standalone sequencing data options.
Shotgun Sample Requirements:
- Total volume of 30uL or more
- Sample eluted into water or dilute Tris/TE buffer
- Sample concentration of greater than:
- 10ng/µL measured by Qubit
- 20ng/µL measured by Nanodrop
What You Will Receive:
- Fastq files (2 x 151bp) for each sample
- Data Analysis and QC Summary
- Materials and Methods Summary
- Data shared via Box folder within 2 weeks.
Offering | Description | Price* |
|---|---|---|
Small Metagenome | Minimum of 6.5M Illumina Reads (1Gbp) with Analysis | $160.00 |
Medium Metagenome | Minimum of 32M Illumina Reads (5Gbp) with Analysis | $345.00 |
Large Metagenome | Minimum of 65M Illumina Reads (10Gbp) with Analysis | $580.00 |
*Orders >48 Samples receive 5% discount
PLEASE SEE OUR FAQ FOR MORE INFORMATION ABOUT MEASUREMENT METHODS