Illumina Sequencing:
The Illumina sequencing packages produce paired end reads (2×151 bp) delivered as fastq files.
For each sample, we require a minimum volume of 30 µL and at least 10 ng/µL dsDNA quantified by Qubit or other dye-based method (20 ng/µL by Nanodrop). Variant calling add-on analysis requires an annotated reference file. DNA extraction services are available for only specific categories of microbes.
Please see our DNA Sequencing: Whole Genome Sequencing page for more detailed information about the package, variant calling analysis, and extraction requirements.
For additional information about variant calling and short read assemblies, please see our Bioinformatics page.