Hybrid Sequencing:
The Nanopore Combo packages combine the high accuracy of Illumina sequencing and long-read length of Nanopore sequencing to close down de novo genomic assemblies. You will receive all raw sequencing fastq files and the annotated assembly in both .gff and .fasta formats.
For each sample, we require 2 aliquots of material per sample:
- One Illumina aliquot: minimum volume of 30 µL, and at least 10 ng/µL dsDNA quantified by Qubit or other dye-based method (20 ng/µL by Nanodrop)
- One Nanopore aliquot: a minimum volume of 60 µL, and at least 40 ng/µL dsDNA quantified by Qubit or other dye-based method (100 ng/µL by Nanodrop)
DNA extraction services are available for only specific categories of microbes. Please see our DNA Sequencing: Hybrid Sequencing page for more detailed information and extraction requirements.